Development of a Full-Length Infectious Cdna Clone of the Grapevine Berry Inner Necrosis Virus.
Identifieur interne : 000080 ( Main/Exploration ); précédent : 000079; suivant : 000081Development of a Full-Length Infectious Cdna Clone of the Grapevine Berry Inner Necrosis Virus.
Auteurs : Xudong Fan [République populaire de Chine] ; Zunping Zhang [République populaire de Chine] ; Fang Ren [République populaire de Chine] ; Guojun Hu [République populaire de Chine] ; Chen Li [République populaire de Chine] ; Baodong Zhang [République populaire de Chine] ; Yafeng Dong [République populaire de Chine]Source :
- Plants (Basel, Switzerland) [ 2223-7747 ] ; 2020.
Abstract
Grapevine berry inner necrosis virus (GINV) belongs to the genus Trichovirus in the family Betaflexiviridae. The GINV isolate LN_BETA_RS was obtained from a "Beta" grapevine (Vitis riparia × Vitis labrusca) exhibiting chlorotic mottling and ring spot in Xingcheng, Liaoning Province, China. To verify the correlation between GINV and grapevine chlorotic mottling and ring spot disease, we constructed an infectious cDNA clone of GINV isolate LN_BETA_RS using the seamless assembly approach. Applied treatments of agroinfiltration infectious cDNA confirmed systemic GINV infection of the Nicotianaoccidentalis 37B by reverse transcription polymerase chain reaction (RT-PCR) and transmission electron microscopy, exhibiting chlorotic mottling symptoms on leaves. Infectious cDNA was also transmitted to new healthy N. occidentalis plants through rub-inoculation. Moreover, the cDNA clone was agroinfiltrated into "Beta" and "Thompson Seedless" grapevine plantlets, and the inoculated grapevines exhibited leaf chlorotic mottling and ringspot during the two years of observation. GINV-inoculated "Beta" grapevines had serious leaf chlorotic mottling and ringspot symptoms on the whole plant, while relatively few symptoms were observed on the leaves of agroinoculated "Thompson Seedless" grapevines in early spring and only weak ring spot gradually appeared later in the top young leaves. Our experiments fulfilled Koch's postulates and revealed the causative role of GINV in grapevine chlorotic mottling and ring spot disease.
DOI: 10.3390/plants9101340
PubMed: 33050558
PubMed Central: PMC7601338
Affiliations:
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when="2020">2020</date><idno type="RBID">pubmed:33050558</idno><idno type="pmid">33050558</idno><idno type="doi">10.3390/plants9101340</idno><idno type="pmc">PMC7601338</idno><idno type="wicri:Area/Main/Corpus">000018</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000018</idno><idno type="wicri:Area/Main/Curation">000018</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000018</idno><idno type="wicri:Area/Main/Exploration">000018</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development of a Full-Length Infectious Cdna Clone of the Grapevine Berry Inner Necrosis Virus.</title><author><name sortKey="Fan, Xudong" sort="Fan, Xudong" uniqKey="Fan X" first="Xudong" last="Fan">Xudong Fan</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100</wicri:regionArea><wicri:noRegion>Xingcheng 125100</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Zunping" sort="Zhang, Zunping" uniqKey="Zhang Z" first="Zunping" last="Zhang">Zunping Zhang</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100</wicri:regionArea><wicri:noRegion>Xingcheng 125100</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Fang" sort="Ren, Fang" uniqKey="Ren F" first="Fang" last="Ren">Fang Ren</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100</wicri:regionArea><wicri:noRegion>Xingcheng 125100</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Guojun" sort="Hu, Guojun" uniqKey="Hu G" first="Guojun" last="Hu">Guojun Hu</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100</wicri:regionArea><wicri:noRegion>Xingcheng 125100</wicri:noRegion></affiliation></author><author><name sortKey="Li, Chen" sort="Li, Chen" uniqKey="Li C" first="Chen" last="Li">Chen Li</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100</wicri:regionArea><wicri:noRegion>Xingcheng 125100</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Baodong" sort="Zhang, Baodong" uniqKey="Zhang B" first="Baodong" last="Zhang">Baodong Zhang</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100</wicri:regionArea><wicri:noRegion>Xingcheng 125100</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Yafeng" sort="Dong, Yafeng" uniqKey="Dong Y" first="Yafeng" last="Dong">Yafeng Dong</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100</wicri:regionArea><wicri:noRegion>Xingcheng 125100</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plants (Basel, Switzerland)</title><idno type="ISSN">2223-7747</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Grapevine berry inner necrosis virus (GINV) belongs to the genus Trichovirus in the family Betaflexiviridae. The GINV isolate LN_BETA_RS was obtained from a "Beta" grapevine (<i>Vitis riparia × Vitis labrusca</i>) exhibiting chlorotic mottling and ring spot in Xingcheng, Liaoning Province, China. To verify the correlation between GINV and grapevine chlorotic mottling and ring spot disease, we constructed an infectious cDNA clone of GINV isolate LN_BETA_RS using the seamless assembly approach. Applied treatments of agroinfiltration infectious cDNA confirmed systemic GINV infection of the <i>Nicotiana</i><i>occidentalis</i> 37B by reverse transcription polymerase chain reaction (RT-PCR) and transmission electron microscopy, exhibiting chlorotic mottling symptoms on leaves. Infectious cDNA was also transmitted to new healthy <i>N. occidentalis</i> plants through rub-inoculation. Moreover, the cDNA clone was agroinfiltrated into "Beta" and "Thompson Seedless" grapevine plantlets, and the inoculated grapevines exhibited leaf chlorotic mottling and ringspot during the two years of observation. GINV-inoculated "Beta" grapevines had serious leaf chlorotic mottling and ringspot symptoms on the whole plant, while relatively few symptoms were observed on the leaves of agroinoculated "Thompson Seedless" grapevines in early spring and only weak ring spot gradually appeared later in the top young leaves. Our experiments fulfilled Koch's postulates and revealed the causative role of GINV in grapevine chlorotic mottling and ring spot disease.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33050558</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>03</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2223-7747</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>10</Issue><PubDate><Year>2020</Year><Month>Oct</Month><Day>11</Day></PubDate></JournalIssue><Title>Plants (Basel, Switzerland)</Title><ISOAbbreviation>Plants (Basel)</ISOAbbreviation></Journal><ArticleTitle>Development of a Full-Length Infectious Cdna Clone of the Grapevine Berry Inner Necrosis Virus.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E1340</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/plants9101340</ELocationID><Abstract><AbstractText>Grapevine berry inner necrosis virus (GINV) belongs to the genus Trichovirus in the family Betaflexiviridae. The GINV isolate LN_BETA_RS was obtained from a "Beta" grapevine (<i>Vitis riparia × Vitis labrusca</i>) exhibiting chlorotic mottling and ring spot in Xingcheng, Liaoning Province, China. To verify the correlation between GINV and grapevine chlorotic mottling and ring spot disease, we constructed an infectious cDNA clone of GINV isolate LN_BETA_RS using the seamless assembly approach. Applied treatments of agroinfiltration infectious cDNA confirmed systemic GINV infection of the <i>Nicotiana</i><i>occidentalis</i> 37B by reverse transcription polymerase chain reaction (RT-PCR) and transmission electron microscopy, exhibiting chlorotic mottling symptoms on leaves. Infectious cDNA was also transmitted to new healthy <i>N. occidentalis</i> plants through rub-inoculation. Moreover, the cDNA clone was agroinfiltrated into "Beta" and "Thompson Seedless" grapevine plantlets, and the inoculated grapevines exhibited leaf chlorotic mottling and ringspot during the two years of observation. GINV-inoculated "Beta" grapevines had serious leaf chlorotic mottling and ringspot symptoms on the whole plant, while relatively few symptoms were observed on the leaves of agroinoculated "Thompson Seedless" grapevines in early spring and only weak ring spot gradually appeared later in the top young leaves. Our experiments fulfilled Koch's postulates and revealed the causative role of GINV in grapevine chlorotic mottling and ring spot disease.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Xudong</ForeName><Initials>X</Initials><Identifier Source="ORCID">0000-0001-9385-1928</Identifier><AffiliationInfo><Affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Zunping</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Fang</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Guojun</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chen</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Baodong</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Yafeng</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>CARS-29-bc-1</GrantID><Agency>the Ministry of Agriculture</Agency><Country></Country></Grant><Grant><GrantID>2018010266-301</GrantID><Agency>Liaoning Development Planning Center</Agency><Country></Country></Grant><Grant><GrantID>2019YFD1001800</GrantID><Agency>Ministry of Science and Technology of the People's Republic of China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Plants (Basel)</MedlineTA><NlmUniqueID>101596181</NlmUniqueID><ISSNLinking>2223-7747</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">GINV</Keyword><Keyword MajorTopicYN="N">grapevine</Keyword><Keyword MajorTopicYN="N">infectious cDNA clone</Keyword><Keyword MajorTopicYN="N">virus</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>09</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>10</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>10</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>14</Day><Hour>1</Hour><Minute>4</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>15</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33050558</ArticleId><ArticleId IdType="pii">plants9101340</ArticleId><ArticleId IdType="doi">10.3390/plants9101340</ArticleId><ArticleId IdType="pmc">PMC7601338</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Arch Virol. 2015 Oct;160(10):2641-5</Citation><ArticleIdList><ArticleId IdType="pubmed">26215445</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 2000;145(10):2217-25</Citation><ArticleIdList><ArticleId IdType="pubmed">11087104</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2017 Jan;101(1):144-149</Citation><ArticleIdList><ArticleId IdType="pubmed">30682318</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2013 May;173(2):286-93</Citation><ArticleIdList><ArticleId IdType="pubmed">23415858</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Microbiol Rev. 1996 Jan;9(1):18-33</Citation><ArticleIdList><ArticleId IdType="pubmed">8665474</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Virol. 2014;58(2):180-4</Citation><ArticleIdList><ArticleId IdType="pubmed">24957724</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 2015 Jul;160(7):1669-78</Citation><ArticleIdList><ArticleId IdType="pubmed">25925705</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 2016 Jul;161(7):2025-7</Citation><ArticleIdList><ArticleId IdType="pubmed">27068163</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2018 Jul;108(7):902-909</Citation><ArticleIdList><ArticleId IdType="pubmed">29436986</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2013 Jan;171(1):15-21</Citation><ArticleIdList><ArticleId IdType="pubmed">23085611</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 2013 Feb;158(2):435-43</Citation><ArticleIdList><ArticleId IdType="pubmed">23081676</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2013 Oct;97(10):1339-1345</Citation><ArticleIdList><ArticleId IdType="pubmed">30722147</ArticleId></ArticleIdList></Reference><Reference><Citation>Viruses. 2019 Jun 04;11(6):</Citation><ArticleIdList><ArticleId IdType="pubmed">31167478</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 2015 Nov;160(11):2661-7</Citation><ArticleIdList><ArticleId IdType="pubmed">26264404</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol Methods. 2009 Feb;155(2):167-74</Citation><ArticleIdList><ArticleId IdType="pubmed">19010356</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2010 Jan;94(1):130</Citation><ArticleIdList><ArticleId IdType="pubmed">30754410</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2019 Mar 19;14(3):e0214010</Citation><ArticleIdList><ArticleId IdType="pubmed">30889228</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2013 Jan;97(1):150</Citation><ArticleIdList><ArticleId IdType="pubmed">30722285</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 1997;142(7):1351-63</Citation><ArticleIdList><ArticleId IdType="pubmed">9267448</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Fan, Xudong" sort="Fan, Xudong" uniqKey="Fan X" first="Xudong" last="Fan">Xudong Fan</name></noRegion><name sortKey="Dong, Yafeng" sort="Dong, Yafeng" uniqKey="Dong Y" first="Yafeng" last="Dong">Yafeng Dong</name><name sortKey="Hu, Guojun" sort="Hu, Guojun" uniqKey="Hu G" first="Guojun" last="Hu">Guojun Hu</name><name sortKey="Li, Chen" sort="Li, Chen" uniqKey="Li C" first="Chen" last="Li">Chen Li</name><name sortKey="Ren, Fang" sort="Ren, Fang" uniqKey="Ren F" first="Fang" last="Ren">Fang Ren</name><name sortKey="Zhang, Baodong" sort="Zhang, Baodong" uniqKey="Zhang B" first="Baodong" last="Zhang">Baodong Zhang</name><name sortKey="Zhang, Zunping" sort="Zhang, Zunping" uniqKey="Zhang Z" first="Zunping" last="Zhang">Zunping 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